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Pseudomonas putida cbb5

Caffeine Junkie: an Unprecedented Glutathione S

Pseudomonas putidaCBB5 was isolated from a soil sample in Coralville, IA, by enriching for microbes capable of utilizing caffeine as a sole carbon and nitrogen source (5). It was found to degrade caffeine and other purine alkaloids by a sequential N-demethylation pathway (6, 7) Pseudomonas putida is a Gram-negative, rod-shaped, saprotrophic soil bacterium. Based on 16S rRNA analysis, P. putida was taxonomically confirmed to be a Pseudomonas species (sensu stricto) and placed, along with several other species, in the P. putida group, to which it lends its name Pseudomonas putida CBB5 was isolated from soil by enrichment on caffeine. This strain used not only caffeine, theobromine, paraxanthine, and 7-methylxanthine as sole carbon and nitrogen sources but also theophylline and 3-methylxanthine Pseudomonas putida CBB5 was isolated from soil by enrichment on caffeine. This strain used not only caffeine, theobromine, paraxanthine, and 7-methylxanthine as sole carbon and nitrogen sources but also theophylline and 3-methylxanthine. Analyses of metabolites in spent media and resting cell suspen

Draft Genome Sequence of the Bacterium Pseudomonas

Proposed sequential N -demethylation of caffeine by

Pseudomonas putida CBB5 breaks caffeine into a carbon dioxide molecule and an ammonia molecule, according to Physorg. No other caffeine eating bacterium has previously been found. It might not seem like an amazing discovery at first, but the ground-breaking find is actually incredibly rare Therefore, bacteria that have evolved to live on caffeine as the sole carbon and nitrogen source are of interest, as are their metabolic pathways toward N -methylated xanthines. Pseudomonas putida CBB5 degrades caffeine, theophylline (1,3-dimethylxanthine), and related methylxanthines via sequential N -demethylation to xanthine (4 - 6)

Pseudomonas putida is a rod-shaped, flagellated, gram-negative bacterium that is found in most soil and water habitats where there is oxygen. It grows optimally at 25-30 C and can be easily isolated Pseudomonas putida is a Gram-negative, rod-shaped, saprotrophic soil bacterium.Based on 16S rRNA analysis, P. putida was taxonomically confirmed to be a pseudomonas species (sensu stricto) and placed, along with several other species, in the P. putida group, to which it lends its name.. Pseudomonas putida je gram-negativní, tyčinkovité, Saprotrofní půdní bakterie.Na základě 16S rRNA analýzy, P. putida byla taxonomicky potvrzeno, že je Pseudomonas druhy (sensu stricto) a umístěn, spolu s několika dalšími druhy, v P. putida skupiny, do které se dodává své jméno.. Pseudomonas putida é unha especie de bacteria gramnegativa con forma de bacilo, saprotrófica que vive no solo.Baseándose en análises de ARNr 16S, confirmouse que P. putida é taxonomicamente unha especie de Pseudomonas (sensu stricto) e foi situada, xunto con outras especies, no grupo P. putida dese xénero, que leva o seu mesmo nome 戀臭假單孢菌(Pseudomonas putida)是一種腐生營養土壤桿菌,屬於格蘭氏陰性菌。基於16S rRNA基因分析,戀臭假單孢菌被分類學上證實為假單胞菌屬(狹義),並連同其他幾個品种放置在戀臭假單孢菌群中,向其中借其名稱。[1] 牠是世界上第一個被賦予專利的生物.

Pseudomonas putida - Wikipedi

Một loài vi khuẩn mới tên Pseudomonas putida CBB5, sử dụng caffein làm nguồn thức ăn đã được phát hiện trong một nghiên cứu tại Đại học Iowa (Mỹ). Một bé gái 5 tuổi ở Trung Quốc bị vi khuẩn ăn mất mũi do mắc phải một că capra 曰く、アイオワ大学の研究チームがカフェインのみを栄養源とする新種の細菌 Pseudomonas putida CBB5 を発見したそうだ (Scientific American の記事、本家 /. 記事より) 。カフェインは炭素、水素、窒素、そして酸素から. Pseudomonas aeruginosa. sitive organisms, Acinetobacter species, or Pseudomonas aeruginosa. tic that has very specific activity against Pseudomonas aeruginosa. bacteria including Bacteroides fragilis and Pseudomonas Pseudomonas putida é um Gram-negativa, em forma de haste saprotrophic solo bactéria.Com base em 16S rRNA análise, P. putida foi taxonomicamente confirmado ser um Pseudomonas espécies (sensu stricto) e colocado, juntamente com várias outras espécies, do P. putida grupo, a que se dá o nome..

Pseudomonas putida jest Gram-ujemnych, w kształcie pręta, saprotrophic gleby bakteria.Na podstawie 16S rRNA analizy P. putida została potwierdzona taksonomicznie być Pseudomonas gatunków (sensu stricto) i umieszcza się, wraz z wieloma innymi gatunkami, w P. putida grupy, do której nadaje jej nazwisko.. Pseudomonas putida è un Gram-negativi, a forma di asta, saprotrophic terreno batterio.Sulla base di 16S rRNA analisi, P. putida è stato confermato essere tassonomicamente una Pseudomonas specie (in senso stretto) e posti, insieme a diverse altre specie in P. putida gruppo, al quale dà il nome.. Pseudomonas putida er en gram-negativ, stavformet, saprotrophic jordbakterien.Basert på 16S rRNA-analyse ble P. putida taksonomisk bekreftet å være en Pseudomonas-art (sensu stricto) og plassert, sammen med flere andre arter, i P. putida-gruppen, som den gir sitt navn til.. Pseudomonas putida is een Gram-negatieve, staafvormige, saprotrophic bodem bacterie.Gebaseerd op 16S rRNA analyse P. putida werd taxonomisch bevestigd dat zijn Pseudomonas species (sensu stricto) en geplaatst, samen met verscheidene andere soorten, in het P. putida groep, waaraan leent zijn naam.. Pseudomonas putida estas Gramnegativa, bastonforma, saprotrofa grund bakterio. Surbaze de 16S rRNA analizo, P. putida estis taksonomie konfirmita esti pseudomonaj specioj (sensu stricto) kaj lokis, kune kun pluraj alia

Pseudomonas putida CR30RNSLL. Pseudomonas putida - пример для продвижения роста завода Rhizobacterium, который производит железо chelating вещества 7 ine alpha,gamma-lyase (MET) gene cloned from Pseudomonas putida. 8 are similar to those of the same enzyme from Pseudomonas putida . 9 olite repression control has been studied in Pseudomonas putida Pseudomonas putida PpG2: The wild-type strain; originally obtained from I. C. Gunsalus, University of Illinois JS388: A PpG2 recA mutant that has been described previously 5 JS390: A PpG2 mutant in which the bkd operon has been deleted between the Nco I sites in bkdR and lpdV and replaced with a tetracycline resistance cassette; construction of this mutant has been described previously

Pseudomonas putida est un Gram-négatif,formetige, saprotrophic sol bactérie.Surbase de 16S ARNr analyse, P. putida a été confirmé comme taxonomiquement une Pseudomonas espèce (stricto sensu) et placé, ainsi que plusieurs autres espèces, dans le P. putida groupe, auquel il donne son nom.. Pseudomonas putida CBB5 is a bacterium that has evolved the ability to metabolize caffeine and other methylxanthines to xanthine using a set of five enzymes, NdmABCDE. NdmABC are N ­ 1 -, N3 -, and N7 -specific N -demethylases that are part of the multicomponent Rieske oxygenase family Pseudomonas putida es un Gram-negativa, con forma de barra saprotrophic suelo bacteria.Sobre la base de 16S rRNA análisis, P. putida se confirmó taxonómicamente ser un Pseudomonas especies (sensu stricto) y colocado, junto con varias otras especies, en la P. putida grupo, a la que presta su nombre..

Two Distinct Pathways for Metabolism of Theophylline and

  1. The University of Alabama The soil bacterium Pseudomonas putida CBB5, first isolated in 2009, is able to metabolize caffeine, theophylline, and related methylxanthines as sole carbon and nitrogen sources 1
  2. The study found that Pseudomonas putida CBB5 uses four newly discovered digestive proteins to break caffeine down into xanthine and then to carbon dioxide and ammonia. It removes the methyl groups from the molecule (
  3. Pseudomonas putida on gramnegatiivne bakter, kelle tüüpiline elukoht on mullas ja kes toitub saprotroofselt. P. putida varieteedid võivad lagundada toksilisi orgaanilisi aineid nagu tolueen [1] ning neid rakendatakse biotervendusel, et lagundada naftat.[2] P. putida eelis teiste Pseudomonas perekonna liikide ees on selle suhteline ohutus.
  4. 四六時中コーヒー飲んでないと生活できない人って(筆者も含めて)時々いますが、このたび見つかった有機溶媒耐性菌(Pseudomonas putida)CBB5は.
  5. Pseudomonas putida CBB5 was isolated from soil by enriching for growth on caffeine (1,3,7-trimethylxanthine). The draft genome of this strain is 6.9 Mb, with 5,941 predicted coding sequences. It includes the previously studied Alx gene cluster encoding alkylxanthine N -demethylase enzymes and other genes that enable the degradation of purine alkaloids
  6. Một loài vi khuẩn mới tên Pseudomonas putida CBB5, sử dụng caffein làm nguồn thức ăn đã được phát hiện trong một nghiên cứu tại Đại học Iowa (Mỹ). Loài vi khuẩn mới sử dụng một enzyme để phá vỡ phân tử caffein, cho.

Pseudomonas putida - WikiMili, The Free Encyclopedi

Pseudomonas putida (n.) 1. A species of gram-negative, aerobic bacteria isolated from soil and water as well as clinical specimens.Occasionally it is an opportunistic pathogen Pseudomonas putida CBB5 uses special enzymes to effectively remove the methyl group, decompose caffeine into CO2 and ammonia and use the energy of this process to grow and develop. This study identified compounds formed during decomposition of caffeine and natural compounds used in asthma medications, improved blood flow and stabilized arrhythmias Pseudomonas putida CBB5 」這個品系可以在純咖啡因中存活,並可將咖啡因降解為二氧化碳和氨 [9] [10]。 參考文獻 外部連結 (英文) Risk Assessment Summary, CEPA 1999. Pseudomonas putida CR30RNSLL(pADPTel) []..

恶臭假单胞菌(Pseudomonas putida)为鱼的一种致病菌,常从腐败的鱼中检出,可作为人类咽部的正常菌群,是人类少见的条件致病菌,偶从人类尿道感染、皮肤感染和骨髓炎标本中分离出,分泌物有腥臭味 NdmB and ndmD genes encoding N-demethylase (Ndm) B and NdmD from Pseudomonas putida CBB5 were successfully cloned in pET32a, designated as pET32a-ndmB-His-His-ndmD (pET32a-BHHD) and electroporated into Escherichia coli strain BL21 (DE3). strain BL21 (DE3) シュードモナス・プチダ カフェインの分解特性 2011年に米アイオワ大学で、P. putida CBB5(遺伝子工学的操作をされていない、土壌から発見された野生株)が、炭素源と窒素源が純粋なカフェインのみの培地上で生育し、カフ.. Pseudomonas putida CBB5 can live on pure caffeine, and has been o Based on 16S rRNA analysis, P. putida has been placed in the P. putida group, to whi P. putida has demonstrated potential biocontrol properti e P450ca Pseudomonas putida a, Gram-negatif, çubuk şeklinde, saprotrofik toprak bakterisi. 16S göre rRNA analizi, P. putida taksonomik bir olduğu doğrulandı Pseudomonas türleri (dar anlamda) ve birçok diğer türlerle birlikte, yerleştirilir, P. putida bu adı katan için, grup..

Pseudomonas putida CBB5, which grows on several purine alkaloids, metabolizes caffeine and related methylxanthines via sequential N-demethylation to xanthine. Metabolism of caffeine by CBB5 was. We have developed a portable caffeine degradation operon by refactoring the alkylxanthine degradation (Alx) gene cluster from Pseudomonas putida CBB5 to function in Escherichia coli. In the process, we discovered that adding a glutathione S -transferase from Janthinobacterium sp. Marseille was necessary to achieve N7 -demethylation activity Pseudomonas putida CBB5 was isolated from soil by enrichment on caffeine. This strain used not only caffeine, theobromine, paraxanthine, and 7-methylxanthine as sole carbon and nitrogen sources but also theophylline and 3-methylxanthine. Analyses of metabolites in spent media and resting cell suspensions confirmed that CBB5 initially N demethylated theophylline via a hitherto unreported. Pseudomonas putida CBB5 NdmA with ferredoxin domain of NdmD Heteromer H9N289; 8 × FES; 6× FE; 2× PG4; 6icm Homology models Oligo-state Ligands QMEAN Template Range Seq id (%) Download Assess -3.43 6icn.1.B. Synonyms: Pseudomonas striata, Pseudomonas sp. UF1/2016, Pseudomonas sp. TC29-1, Pseudomonas sp. TAU2/2016, Pseudomonas sp. PX-A-Ps.sp2, Pseudomonas sp. PT1/2016.

Izolovali jsme novou bakterii degradující kofein, Pseudomonas putida CBB5, který rozkládá kofein na oxid uhličitý a čpavek, říká Ryan Summers, který svůj výzkum představil na 111. generální schůzi Americké společnosti pr Pseudomonas putida CBB5 was isolated from soil by enriching for growth on caffeine (1,3,7-trimethylxanthine). The draft genome of this strain is 6.9 Mb, with 5,941 predicted coding sequences. It includes the previously studied Alx.

Two distinct pathways for metabolism of theophylline and

Draft Genome Sequence of the Bacterium Pseudomonas putida CBB5, Which Can Utilize Caffeine as a Sole Carbon and Nitrogen Source Erik M. Quandt,a Ryan M. Summers,b Mani V. Subramanian,c Jeffrey E. Barricka. Pseudomonas putida CBB5 degrades caffeine, theophylline (1,3-dimethylxanthine), and related methylxanthines via sequen- tial N -demethylation to xanthine ( 4-6 )

The different strains affiliated so far to P. putida were retrieved in different Pseudomonas clades, with P. 'putida' FDAARGOS121 branching within the P. aeruginosa lineage, and P. 'putida' CBB5 and MC4 5222 within the group Pseudomonas putida CBB5 NdmB homo-6-mer 6 × FES; 6× FE; 6icl Cartoon Cartoon Tube Trace Lines Ball+Stick Licorice Hyperball Rope Surface Spacefill Outline Fog Background Transparent Resolution Low Medium High.

Pseudomonas putida - Pseudomonas putida - qwe

Draft Genome Sequence of the Bacterium Pseudomonas putida CBB5 that can Utilize Caffeine as a Sole Carbon and Nitrogen Source. Quandt E.M., Summers R.M., Subramanian M.V., Barrick J.E. Submitted (NOV-2014) to th You're still no match for a newly described bitty bacteria called Pseudomonas putida CBB5. These little guys can feast on pure caffeine all day—and presumably all night—long. And researchers. Pseudomonas Pseudomonas aeruginosa Pseudomonas putida Pseudomonas fluorescens Pseudomonas syringae Pseudomonas Phages Pseudomonas stutzeri Escherichia coli Pseudomonas mendocina Bacteria Gram-Negativ Two distinct pathways for metabolism of theophylline and caffeine are coexpressed in Pseudomonas putida CBB5. J Bacteriol. 2009; 191(14):4624-32 (ISSN: 1098-5530 Pseudomonas putida CBB5 was isolated from soil by enrichment on caffeine. This strain used not only caffeine, theobromine, paraxanthine, and 7-methylxanthine as sole carbon and nitrogen sources but also theophylline and 3.

Pseudomonas putida - meddi

Read about IDT products used in research, get expert application advice, and find answers to common research questions. Caffeine addicted bacteria—an iGEM project Quandt EM, Hammerling MJ, et al. (2013) Decaffeination and measurement of caffeine content by addicted Escherichia coli with a refactored N-demethylation operon from Pseudomonas putida CBB5 Involved in the caffeine degradation, which is the essential first step for assimilating the carbon and nitrogen in caffeine. Catalyzes the N3-demethylation of theobromine to produce 7-methylxanthine and formaldehyde. Also catalyzes. A non-heme iron oxygenase. The enzyme from the bacterium Pseudomonas putida prefers NADH over NADPH. The enzyme is specific for 7-methylxanthine . Forms part of the caffeine degradation pathway Недавно обнаруженная бактерия Pseudomonas putida CBB5 весьма активно им питается и вполне способна жить вообще за счёт одного только кофеина. Pseudomonas putida - бактерия, способная жить на кофеине

Abstract A novel caffeine-degrading bacterium, Pseudomonas putida CBB5 was isolated from the soil by an enrichment procedure using caffeine as the sole source of carbon and nitrogen. CBB5 grew not only on caffeine. The caffeine degradation pathway of Pseudomonas putida CBB5 utilizes an unprecedented glutathione-S-transferase-dependent Rieske oxygenase for demethylation of 7-methylxanthine to xanthine, the final step in caffeine Izolirali smo novu bakteriju koja razgrađuje kofein, Pseudomonas putida CBB5, koji razgrađuje kofein u ugljični dioksid i amonijak, kaže Ryan Summers, koji je predstavio svoja istraživanja na 111. skupštini Američkog društva z

Pseudomonas putida CBB5 NdmA with ferredoxin domain of NdmD Released: 04 Sep 2019 Source organism: Pseudomonas putida Primary publication: Structural and Mechanistic Insights into Caffeine Degradation by the Kim JH. The widespread use of caffeine (1,3,7-trimethylxanthine) and other methylxanthines in beverages and pharmaceuticals has led to significant environmental pollution. We have developed a portable caffeine degradation operon by refactoring the alkylxanthine degradation (Alx) gene cluster from Pseudomonas putida CBB5 to function in Escherichia coli. In the process, we discovered that adding a. It turns that CBB5 as they are better known need their daily java similar to 70% of us. In fact, they thrive on it. This is a new bacteria found by the Meet your coffee friend -Pseudomonas Putida CBB5 by chee Leng (on coffee. Proposed caffeine N-demethylation pathway (A) and map of associated genes (B) in Pseudomonas putida CBB5. The dashed arrows The dashed arrows in part (A) represent a minor pathway, accounting for 1-2% of metabolized caffeine caffeine-degrading bacterium, Pseudomonas putida CBB5. By successive N-demethylation, the bacterium builds the intact pathway to degrade caffeine and other related methylxanthines [11,12]. Summers et al. [13] have identifie

恋臭假单孢菌(Pseudomonas putida)是一种腐生营养土壤杆菌,属于格兰氏阴性菌。基于16S rRNA基因分析,恋臭假单孢菌被分类学上证实为假单胞菌属(狭义),并连同其他几个品种放置在恋臭假单孢菌群中,向其中借其名称 Abstract NdmB genes from Pseudomonas putida CBB5 and GO genes from spinach, which encode N-demethylase B (NdmB) and Glycolate oxidase (GO) respectively, were separately ligated into expression vectors of pACYCDuet-1 and pET32a to construct recombinant plasmids of pACYCDuet-1-ndmBHis (pBH) and pET32a-GOHis (pGOH). ). Then the two plasmids were both transformed in Escherichia coli (E. coli. Ternyata Anda belum ada apa-apanya dibanding Pseudomonas putida CBB5, sebuah bakteri yang baru ditemukan. Bakteri ini hidup dari kafein dan kini peneliti berhasil mengetahui bagaimana mereka bisa melakukan itu

Kaca-Kaca: Pseudomonas Putida CB55, Bakteri Pengkonsumsi

Pseudomonas putida (ID 174) - Genome - NCB

FMN-binding fluorescent protein (1,460 words) exact match in snippet view article find links to article Basis for the Slow Dark Recovery of a Full-Length LOV Protein from Pseudomonas putida.J Mol Biol. 417 (4): 362-374. doi:1 Pseudomonas putida CBB5 uses special enzymes to break that caffeine down into carbon dioxide and ammonia, generating energy in the process. Within the caffeine molecule are three structures, known. Title: Pseudomonas putida, Author: Valerie Ho, Name: Pseudomonas putida, Length: 21 pages, Page: 1, Published: 2013-12-13 Issuu company logo Close Stories Discover Categories Become a Publisher.

Pseudomonas Putida CBB5: New Bacteria Can Live On

www.tripolicastle.com mersall Pseudomonas putida CBB5 posee enzimas capaces de formar dióxido de carbono y amonio a partir de cafeína (que está formada básicamente por carbono, hidrógeno, nitrógeno y oxígeno. Ya véis que no es para tanto)

Bakteri tersebut dinamakan Pseudomonas putida CBB5 yang bisa mengubah kafein menjadi CO2 dan amonia. Bakteri tersebut bisa hidup dalam lingkungan dengan kadar kafein 2,5 gram/liter, yang bagi banyak bakteri suda Pseudomonas putida CBB5 genome sequencing Whole Genome Sequencing Pseudomonas putida 4.5G BioProject 17 SRP049998 SRA201934 Bacterial isolates from intensive care units Other Achromobacter xylosoxidans. Pseudomonas Pseudomonas aeruginosa Bacterias Pseudomonas putida Pseudomonas fluorescens Bacterias Gramnegativas Bacterias Grampositivas Pseudomonas syringae Pseudomonas stutzeri Fagos Pseudomonas Olea 1. Pseudomonas putida CBB5 was isolated from soil by enrichment on caffeine. This strain used not only caffeine, theobromine, paraxanthine, and 7-methylxanthine as sole carbon and nitrogen sources but also theophylline and

Plenty of incidents of improper usage of propranolol

Synchrotron SAXS data from solutions of Pseudomonas putida CBB5 NdmAB complex - static in 20 mM HEPES 150 mM NaCl 2 mM TCEP 10% v/v glycerol, pH 7.5 were collected on the 4C beam line at the Pohang. The strain Pseudomonas putida CBB5 was isolated from soil and found to catabolize caffeine by the mechanism described above using N-demethylases. The genes encoding enzymes required for caffeine degradation are related to a specific transmissible plasmid found in caffeine-resistant strains of Pseudomonas

Pseudomonas putida NCIMB 9866 The pch gene cluster follows the protocatechuate branch of the ortho pathway of p -cresol degradation, encoding for the enzymes p -cresol methylhydroxylase and p -hydroxybenzaldehyde dehydrogenase, which degrade p -cresol to p -hydroxybenzoic acid [4] Characterization of a broad-specificity non-haem iron N-demethylase from Pseudomonas putida CBB5 capable of utilizing several purine alkaloids as sole carbon and nitrogen source. Microbiology. 2011; 157(Pt 2):583-59 Quandt, E. M. et al. Decaffeination and measurement of caffeine content by addicted Escherichia coli with a refactored N-demethylation operon from Pseudomonas putida CBB5. ACS Synth. Biol. 2, 301. 戀臭假單孢菌(Pseudomonas putida)是一種腐生營養土壤桿菌,屬於格蘭氏陰性菌。基於16S rRNA基因分析,戀臭假單孢菌被分類學上證實為假單胞菌屬(狹義),並連同其他幾個品种放置在戀臭假單孢菌群中,向其中借其名稱 You're still no match for a newly described bitty bacteria called Pseudomonas putida CBB5. These little guys can feast on pure caffeine all day—and presumably all night—long. And researchers have now located just how the

SASDFH7 - Pseudomonas putida CBB5 NdmAB complex Methylxanthine N1-demethylase NdmA Methylxanthine N3-demethylase NdmB Download files - MW experimental 227 kDa MW expected 256 kDa log I(s) 2.72×10 0 -1. A non-heme iron oxygenase. The enzyme from the bacterium Pseudomonas putida shares an NAD(P)H-FMN reductase subunit with EC 1.14.13.179, methylxanthine N3-demethylase, and has a Pseudomonas putida CBB5 was isolated from soil by enriching for growth on caffeine (1,3,7-trimethylxanthine). The draft genome of this strain is 6.9 Mb, with 5,941 predicted coding sequences. It includes the previously studie Pseudomonas Putida CBB5 Live Science: What a Jolt! Microbe Lives on Caffeine Posted by Jim Sande at 8:50 AM No comments: Post a Comment Newer Post Older Post Home Subscribe to: Post Comments (Atom) Jim Sande. Sigma-Aldrich offers abstracts and full-text articles by [Chi Li Yu, Tai Man Louie, Ryan Summers, Yogesh Kale, Sridhar Gopishetty, Mani Subramanian]. ADVANCED SEARC

In the coding sequence of P. putida LLL is the most abundant tripeptide. [8] 有機合成 [编辑] CBB5和分解咖啡因 [编辑] 「Pseudomonas putida CBB5」這個品系可以在純咖啡因中存活,並可將咖啡因降解為二氧化碳和氨 [9] [10] Bakteria ta to Pseudomonas putida CBB5, rozkłada ona kofeinę przy pomocy enzymów trawiennych, które były przedmiotem badań. Ta zdolność pozwala bakterii żyć i rozwijać się. W jaki sposób bakteria potrafi rozkłada Pseudomonas putida CBB5 namanya, ia adalah bakteri yang dapat bertahan hidup hanya pada kafein. Peneliti dari University of Texas, Jeffrey E. Barrick, mempercayai jika bakteri kecanduan kafein ini bisa digunakan sebagai obat bioproduction di daerah yang terkontaminasi air limbah (dapat digunakan untuk membersihkan lingkungan yang terkontaminasi)

Nueva bacteria descubierta se alimenta de café10 Surprising Things That Bacteria Like to EatRYAN M SUMMERS | University of Alabama, Alabama | UA

327764 Microbial Caffeine Junkies: Growth of Pseudomonas Putida CBB5 On Caffeine and Metabolic Engineering of E. Coli for Production of High-Value Methylxanthines From Caffeine Sunday, November 3, 201 A non-heme iron oxygenase. The enzyme from the bacterium Pseudomonas putida shares an NAD(P)H-FMN reductase subunit with EC 1.14.13.178, methylxanthine N1-demethylase, and has higher activity with NADH. The scientists knew that a natural soil bacterium, Pseudomonas putida CBB5, can actually live solely on caffeine and could be used to clean up such environmental contamination. So they set out to transfer genetic gear for metabolising, or breaking down, caffeine from P putida into that old workhorse of biotechnology, E coli, which is easy to handle and grow 次の論文は Scholar 内で結合されています。結合された引用は、最初の論文のみがカウントされます。 この [引用先] の件数には Scholar 内の次の論文への引用も含まれています。* が付いた論文は、プロフィール内の論文とは異なる場合があります Quandt, E.M. et al. Decaffeination and measurement of caffeine content by addicted Escherichia coli with a refactored N-demethylation operon from Pseudomonas putida CBB5. ACS Synth. Biol. in press. 285

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